Translational_Unit

Part:BBa_K1033120:Design

Designed by: Magnus Bergman, Pontus Danielsson, Alexander Wirtanen, Nils Anlind, Christoffer Frisk, Thorsteinn Olaufsson, Malin Borg, Niclas Sandberg   Group: iGEM13_Uppsala   (2013-09-02)


B0034-ExpTag-Linker-Miraculin-Linker-6xHis


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The miraculin sequence is codon optimized for E.coli. The codon usage for the export tag USP45's is best suited for Lactobacillus strains


Source

Richadella Dulcifica, but codon optimized for E.coli only the miraculin is codon optimized. The codon usage for the export tag USP45's is best suited for Lactobacillus strains

References